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Human-CD8+-Cytotoxic-Killer-T-Cells

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Human-CD8+-Cytotoxic-Killer-T-Cells

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	Print Version
Description	Human CD8+ Cytotoxic T cells are a sub-group of T lymphocytes that are capable of inducing the death of infected somatic [with viruses or other pathogens] or tumor cells. Most CD8+ Cytotoxic T cells express T-cell receptors (TCRs) that can recognize a specific antigenic peptide bound to Class I MHC molecules (present on all nucleated cells) and a glycoprotein called CD8. The MHC molecule keeps the Cytotoxic T cell and the target cell bound closely together during antigen-specific activation. CD8+ T cells are recognized as Cytotoxic T cells once they become activated and are generally classified as having a pre-defined cytotoxic role within the immune system.
Procedure Overview
BioSafety Level	II
Organism	Homo sapiens
Isolation	These cells are extracted from whole blood using Lymphoprep(R), a hydrophilic polysaccharide that separates layers of blood, with monocytes and lymphocytes forming a buffy coat under a layer of plasma.
Growth Properties	Suspension
Morphology	Spheric
Passage Number	They are cryopreserved immediately after isolation
Population Doubling	The Human CD8+ Cytotoxic Killer T Cells do not proliferate in vitro.
Markers	CD8+
Applications	For Research Use Only. The most common applications of CD8+ Cytotoxic T cells are ELISPOT and Cytotoxicity assays.
Disease	Normal
Propagation	The basal medium for this cell line is Prigrow X series medium available in ABM, Cat. No. TM4121. Atmosphere: air, 95%; Carbon dioxide (CO₂), 5%. The Human CD8+ Cytotoxic Killer T Cells do not proliferate in vitro.
Quality Control	ABM provides cryopreserved animal hepatocytes from a large variety of clinical species. Every lot of our cryopreserved hepatocytes is characterized for viability, stability, attachment efficiency, monolayer confluency and drug metabolizing enzyme activity
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.