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Rabbit-Primary-Peripheral-Blood-Mononuclear-Cells

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Rabbit-Primary-Peripheral-Blood-Mononuclear-Cells

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Rabbit-Primary-Peripheral-Blood-Mononuclear-Cells

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	Print Version
Description	The New Zealand White Rabbit Peripheral Blood Mononuclear Cells (PBMC) consists of lymphocytes and monocytes, which can be used for a variety of immunology applications/assays. The rabbit PBMCs secret cytokines and chemokines to regulate immune response, as well as, provide essential growth supplement for growing rabbit primary cells. These cells are useful in the fields of immunology, infectious disease and autoimmune responses.
Procedure Overview
BioSafety Level	II
Organism	Oryctolagus cuniculus (New Zealand White Rabbit)
Source Organ	Blood
Isolation	These cells are extracted from buffy coats using Ficoll-Paque^TM PLUS, sterile Ficoll sodium diatrizoate solution, which separates layers of blood, with monocytes and lymphocytes forming a buffy coat under a layer of plasma.
Growth Properties	Suspension
Morphology	Spheric
Applications	For Research Use Only
Disease	Normal
Propagation	The basal medium for this primary cell is Prigrow II medium available from abm, Cat. No. TM002. To make the complete growth medium, add the following components to the basal medium: 10% fetal bovine serum (TM999) 1% (v/v) sodium pyruvate, 2 mM L-Glutamine and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO₂), 5%. The Rabbit PBMCs do not proliferate in vitro. To prepare supernatant of cultures of activated T cells, dilute PBMC to 0.5×10⁶ cells per mL in complete growth medium and stimulate with Concanavalin A (10 ug/mL) and PMA (20 ng/mL). Harvest supernatant on days 2, 4 and 6. To prepare for PBMC plasma cell ELISPOT assay, dilute PBMC to desire concentration and culture in complete growth medium with 0.5 mM 2-Mercaptoethanol (CH044) overnight.
Subculturing	The PBMCs do not proliferate in vitro
Reference	Schrader, JW 2015. Methods of isolating cells and generating monoclonal antibodies, US Patent 8945857.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.