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Mouse-Primary-Cochlear-Endothelial-Cells

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Mouse-Primary-Cochlear-Endothelial-Cells

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Mouse-Primary-Cochlear-Endothelial-Cells

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Description	As part of the fluid-blood barrier in the stria vascularis, the cochlear microvascular endothelial cells, along with perivascular resident macrophate-type melanocytes (PVM/Ms) and cochlear pericytes, are important for maintaining the inner ear homeostasis. The cochlear microvascular endothelial cells form tight junctions and primarily sustains the barrier integrity. With all three primary cells available, co-culture of the cell types is useful in studying the underlying causes of vascular dysfunction-related hearing loss.
Procedure Overview
BioSafety Level	II
Organism	C57BL/6J mice
Source Organ	Cochleae
Isolation	Mouse Primary Cochlear Endothelial Cells were isolated and purified by flow cytometry based on isolectin B4 (BSI-B4) sorting. Sorted cells were validated with anti-von Willebrand factor (vWF) antibody in immunohistochemical staining.
Growth Properties	Adherent
Morphology	Flat, elongated and generally cuboidal morphology
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Markers	vWF
Applications	For Research Use Only
Donor Age	10- to 15-day-old
Propagation	Use of PriCoat? T25 Flasks (G299) or . Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The basal medium for this cell line is CS-C Medium (Sigma, Cat No.C1556). To make the complete growth medium, add the following components to the basal medium: 10% fetal bovine serum (TM999), 1% endothelial cell growth factor (Sigma, Cat. No. E9640) and Penicillin/Streptomycin (G255). Atmosphere: air, 95%; Carbon dioxide (CO2), 5%; Temperature: 37.0°C.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.