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HeLa Overexpressing Stable tCD-Luc2 Cell Line

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HeLa Overexpressing Stable tCD-Luc2 Cell Line

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HeLa Overexpressing Stable tCD-Luc2 Cell Line

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	Print Version
BioSafety Level	II
Organism	Homo sapiens
SourceOrgan	Cervix
Growth Properties	Adherent
Morphology	Polygonal
Description	The HeLa Overexpressing Stable tCD-Luc2 Cell Line contains the fusion gene tCD2-luc2 from the luciferase 2 protein and the rat CD2 protein. The overexpression of tCD2-luc2 in the HeLa cells was done by stable transfection using lentiviral infection with the plasmid DNA containing the fusion gene. The growth of this cell line is identical to the wild type cells which indicates that the exogenous protein tCD2-luc2 has no toxic effect. The HeLa Overexpressing Stable tCD-Luc2 Cell Line shows a significant amount of luciferase activity which produces bioluminescent cells. This is useful as an experimental tool of major importance for cancer investigation, especially imaging of tumors in xenografted animals where stable GFP, labelling of tumor cells have to be avoided.
Procedure Overview
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Over Expression	tCD2-luc2 gene introduced via lentiviral infection
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from abm(TM003). To make the completed growth medium, add the following components to the base medium: Fetal Bovine Serum (TM999) to final concentration of 10% and 1% Penicillin/Streptomycin Solution. Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
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Quality Control	1) Flow cytometry on the basis of OX34 staining; 2) Western blot analysis of tCD2-luc2
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.