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Cas9 Expressing RCS Cell Line

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Cas9 Expressing RCS Cell Line

商品詳情參考文獻相關(guān)資料

	Print Version
BioSafety Level	II
Organism	Rat
SourceOrgan	Spine
Growth Properties	Suspension/Adherent
Morphology	Round
Description	Swarm rat chondrosarcoma (RCS) cells were derived from neoplasm in rat spine and is a model for chondrosarcoma tumor development. The Cas9 expressing RCS Cell Line was established through co-transfection with hCas9_P2A_mCherry_T2a_Puro and pOG44 vector. The generation of the Cas9 expressing RCS cell line allows users to generate targeted mutations in genes via transfection or lentiviral transfection with single-guide RNAs (sgRNAs). This cell line is a valuable research tool which enables users endless possibilities to design their desired cell line for the study of chondrocytes and gene function.
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	Puromycin
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, 3 μg/ml puromycin for selection, and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Can't find what you want? Take advantage of our custom service: WT Control Cell Line Expressing Cas9 for Comparison and Genome-wide CRISPR Knockout cell lines
Disclaimer	1. The end user acknowledges that the Materials provided under abm’s Material Transfer Agreement (MTA) does not grant a license for commercial use or imply any ownership rights of any intellectual property rights relating to the Materials. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for functional freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 5. Please note that if the gene to be knockout may be essential to cell survival, abm will provide a hemizygous pool as the default deliverable to compensate for any lethal effects (unless otherwise requested).