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EACH-1 Cells

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EACH-1 Cells

商品詳情參考文獻(xiàn) 相關(guān)資料

	Print Version
BioSafety Level	II
Organism	Human
Source Organ	Posterior scapular spine
Disease	Parachordoma
Donor Age	28
Donor Gender	Male
Growth Properties	Adherent
Morphology	Polygonal
Population Doubling	Doubling time of 37 hours
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	Vimentin, S-100, AE1/AE3, p63 (negative)
Applications	For Research Use Only
Description	EACH-1 Cells are novel extra-axial chordoma tumor cells which exhibit chromosomal aberrations, and are tumorigenic and can form colonies on soft agar. It may be used to facilitate studies on the development and treatment of the rare low grade malignant tumor.
Procedure Overview
Propagation	The base medium for this cell line is Prigrow III medium available from abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10% and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	Flow cytometry was used to identify cell surface markers
Reference	Decomas, A. M., Penfornis, P., Harris, M. R., Meyer, M. S., & Pochampally, R. R. (2010). Derivation and Characterization of an Extra-Axial Chordoma Cell Line (EACH-1) from a Scapular Tumor. The Journal of Bone and Joint Surgery-American Volume, 92(5), 1231-1240. doi:10.2106/jbjs.i.00594
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.