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CUTLL1 Cells

品牌：Abmgood

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CUTLL1 Cells

商品詳情參考文獻(xiàn) 相關(guān)資料

	Print Version
BioSafety Level	II
Organism	Human
Source Organ	Blood
Disease	T-cell lymphoma
Growth Properties	Suspension
Morphology	Round
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Description	CUTLL1 Cells are derived from human T-cell lymphoblastic leukemia (T-ALL) which expresses high levels of NOTCH1. NOTCH1 is an important target in cancer therapies in which inhibition has potential therapeutic significance. The CUTLL1 cells have genetic aberrations with t(7;9)(q34;q34) translocation which increases the NOTCH1 signaling. It is a valuable model for cancer research.
Procedure Overview
Propagation	The base medium for this cell line is Prigrow II medium available from abm(TM002). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 20% and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Reference	Palomero, T., Barnes, K. C., Real, P. J., Bender, J. L., Sulis, M. L., Murty, V. V., . . . Ferrando, A. A. (2006). CUTLL1, a novel human T-cell lymphoma cell line with t(7;9) rearrangement, aberrant NOTCH1 activation and high sensitivity to γ-secretase inhibitors. Leukemia, 20(7), 1279-1287. doi:10.1038/sj.leu.2404258
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.