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mNANOG Purified Protein

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mNANOG Purified Protein

商品詳情參考文獻(xiàn) 相關(guān)資料

	Print Version
Organism	Mouse
Accession #	Q80Z64
Alias	Ecat4, Enk
Protein Type	Wild Type
Protein Sequence	MSVGLPGPHSLPSSEEASNSGNASSMPAVFHPENYSCLQGSATEMLCTEAASPRPSSEDLPLQGSPDSSTSPKQKLSSPEADKGPEEEENKVLARKQKMRTVFSQAQLCALKDRFQKQKYLSLQQMQELSSILNLSYKQVKTWFQNQRMKCKRWQKNQWLKTSNGLIQKGSAPVEYPSIHCSYPQGYLVNASGSLSMWGSQTWTNPTWSSQTWTNPTWNNQTWTNPTWSSQAWTAQSWNGQPWNAAPLHNFGEDFLQPYVQLQQNFSASDLEVNLEATRESHAHFSTPQALELFLNYSVTPPGEI
Protein Family	Belongs to the Nanog homeobox family.
Functions	Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. When overexpressed, promotes cells to enter into S phase and proliferation. Nanog is one of the four factors in the Thomson protocol for iPSC reprogramming. The recombinant protein within the lysate has a 6X His tag at N-terminus for purification and 9X Arginine at C-terminus for cell membrane penetration.
Cell Types	Unknown
Protein Location	Nucleus.
Tag	6X His tag at N-terminus and 9X Poly-Arginine at C-terminus
Form	Liquid
Expression System	E.coli
Type	Purified Protein
Purification	Affinity Chromatography
Quality Control	By Western Blot Analysis
Concentration	250ug/ml
Storage	PBS
Shipping	On dry ice.
Notes	For Research Use Only, not for therapeutic or diagnostic purposes.
References	Induced pluripotent stem cell lines derived from human somatic cells. Yu et al. Science. 2007 Dec 21;318(5858):1917-20. Epub 2007 Nov 20. Human induced pluripotent stem cells free of vector and transgene sequences. Yu et al. Science. 2009 May 8;324(5928):797-801. Epub 2009 Mar 26.