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NANOG Purified Protein

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NANOG Purified Protein

商品詳情參考文獻相關(guān)資料

	Print Version
Organism	Human
Accession #	Q9H9S0
Alias	Homeobox protein NANOG
Protein Type	Wild Type
Protein Sequence	HHHHHHSVDPACPQSLPCFEASDCKESSPMPVICGPEENYPSLQMSSAEMPHTETVSPLPSSMDLLIQDSPDSSTSPKGKQPTSAEKSVAKKEDKVPVKKQKTRTVFSSTQLCVLNDRFQRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKSKRWQKNNWPKNSNGVTQKASAPTYPSLYSSYHQGCLVNPTGNLPMWSNQTWNNSTWSNQTQNIQSWSNHSWNTQTWCTQSWNNQAWNSPFYNCGEESLQSCMQFQPNSPASDLEAALEAAGEGLNVIQQTTRYFSTPQTMDLFLNYSMNMQPEDVESGGGGSPGRRRRRRRRR
Protein Family	Belongs to the Nanog homeobox family.
Functions	? Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Nanog imposes pluripotency on ES cells and prevents their differentiation towards extra-embryonic endoderm and trophectoderm lineages. It also blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. When NANOG is overexpressed, it promotes cells to enter into S phase and proliferation. ? NANOG is one of the four factors in the Thomson protocol for iPSC reprogramming. The recombinant protein within the lysate has a 6X His tag at N-terminus for purification and 9X Arginine at C-terminus for cell membrane penetration.
Cell Types	Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
Protein Location	Nucleus.
Tag	6X His tag at N-terminus and 9X Poly-Arginine at C-terminus
Form	Liquid
Expression System	E.coli
Type	Purified Protein
Purification	Affinity Chromatography
Quality Control	By Western Blot Analysis
Concentration	250ug/ml
Storage	PBS
Shipping	On dry ice.
Notes	For Research Use Only, not for therapeutic or diagnostic purposes.
References	Induced pluripotent stem cell lines derived from human somatic cells. Yu et al. Science. 2007 Dec 21;318(5858):1917-20. Epub 2007 Nov 20. Human induced pluripotent stem cells free of vector and transgene sequences. Yu et al. Science. 2009 May 8;324(5928):797-801. Epub 2009 Mar 26.