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Immortalized-Human-Osteoprecursor-Cells-(OPC1)

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Immortalized-Human-Osteoprecursor-Cells-(OPC1)

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Immortalized-Human-Osteoprecursor-Cells-(OPC1)

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	Print Version
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Bone
Donor Age	12-13 weeks, fetal
Growth Properties	Adherent
Morphology	Polygonal
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	Alkaline phosphatase (ALP), osetocalcin (OC), osteonectin (ON), osteopontin (OP), procollagen-I (ProI), and PTH receptor (PTHR)
Applications	For Research Use Only
Immortalization Method	Serial passaging and calcium phosphate-mediated transfection with a plasmid carrying SV40 T antigen, activated by human A/D interferon
Description	The Immortalized Human Osteoprecursor Cells (OPC1) is a conditionally immortalized fetal osetoprecusor cell line uniquely expressing SV40 Tag in the presence of human interferon A/D which affects the proliferation capability. However, the Tag may be expressed constitutively in the presence of serum. It expresses alkaline phosphatase (ALP), osetocalcin (OC), osteonectin (ON), osteopontin (OP), procollagen-I (ProI), and PTH receptor (PTHR). The cells are able to differentiate in the presence of recombinant human BMP-2 (rhBMP2), a regulatory factor involved in differentiation. This cell line is valuable tool as a model system in studies in bone-graft substitutes.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is PriGrow VIII, available at abm, Cat. No. TM018. To make the completed growth medium, add the following components to the base medium: 5% fetal bovine serum (TM999), 750 U/ml human A/D interferon (R&D Systems), 1% Penicillin/Streptomycin (G255), and 0.2 mg/ml G418 for selection. Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C. To differentiate add rhBMP2 at 10 ng/ml.
Quality Control	1) RT-PCR analysis for osteoblast markers; 2) ALP staining was utilized to determine presence of ALP
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.