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Immortalized-Human-Lymphoblastoid-Cells-–-EBV-(KMS-15)

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Immortalized-Human-Lymphoblastoid-Cells-–-EBV-(KMS-15)

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Immortalized-Human-Lymphoblastoid-Cells-–-EBV-(KMS-15)

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	Print Version
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Peripheral blood
Growth Properties	Suspension
Morphology	Lymphoblast-like
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Immortalization Method	Epstein-Barr Virus (EBV)
Description	The Immortalized Human Lymphoblastoid Cells- EBV (KMS-15) was derived from peripheral human blood lymphocytes, with minimal genetic and phenotypic alterations compared to the parental lymphocytes. Being an actively proliferating B cell population, these cells provide a constant supply of starting materials suitable for various assays and in vitro model system for the study of human B cells. The KMS-15 cells are positive for both CD19 and CD20 B cell antigens as well as HLA-DR. In contrast, another lyphoblastoid cell line, KMS-9 (Cat#T0132) is positive for HLA-DR and CD19B while tested negative for CD20.
Procedure Overview
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Propagation	The base medium for this cell line is Prigrow II medium available from ABM (TM002). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10% and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.