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Immortalized-Human-Mammary-MUC+ESA+-Epithelial-Cells-

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Immortalized-Human-Mammary-MUC+ESA+-Epithelial-Cells-

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Immortalized-Human-Mammary-MUC+ESA+-Epithelial-Cells-

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	Print Version
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Mammary Gland
Donor Age	22
Donor Gender	Female
Growth Properties	Adherent
Morphology	Polygonal
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	CK18, E-cadherin
Applications	For Research Use Only
Immortalization Method	Serial passaging and transduction with human papilloma virus (HPV)-16 E6/E7
Description	Immortalized Human Mammary MUC+ESA+ Epithelial Cells are luminal-derived epithelial cells coexpressing sialomucin (MUC) and epithelial-specific antigen (ESA). It has tight junction proteins, occludin, claudin-1, and the ability to build high transepithelial electrical resistance which is all characteristics of differentiated luminal epithelial phenotypes. It can form acinus-like spheres when cultures on laminin rich gel. These cells have characteristics of differentiated mature human breasts.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is PriGrow III: Prigrow IV (3:1 ratio) medium available from ABM (TM003 and TM004). To make the completed growth medium, add the following components to the base medium to final concentration of: 0.5 μg/ml hydrocortisone, 50 μg/ml gentamicin (G272), 5 μg/ml insulin (Z101065), 10 ng/ml EGF (Z100135), 10-10 M cholera toxin, 1.8 x 10-4 M adenine, 10 μM Y27632, 2 mM glutamine, 5% FCS and Penicillin/Streptomycin Solution (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	Immunocytochemical staining used to determine cell traits
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.