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Immortalized-(Conditionally)-Rat-Decidual-Cells-(GG-AD)

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Immortalized-(Conditionally)-Rat-Decidual-Cells-(GG-AD)

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Immortalized-(Conditionally)-Rat-Decidual-Cells-(GG-AD)

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	Print Version
BioSafety Level	II
Organism	Sprague-Dawley Rat
Source Organ	antimesometrial decidua
Growth Properties	Adherent
Morphology	Binucleated, small and compact at permissive temperature; polynucleated, differentiated and flat at non-permissive temperature.
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	IL-6, IL-6R, gp130, activin βA, decidual PRL-related protein (dPRP)
Applications	For Research Use Only
Immortalization Method	Serial passaging and infection by SV-40 tsA209 Temperature Sensitive Mutant virus
Description	The decidual cells play an important role in the maintenance of pregnancy at the maternofetal interface after implantation of the fertilized egg into the endometrium. The Immortalized Rat Decidual Cells (GG-AD) is conditionally immortalized through the temperature-sensitive simian virus (SV40 tsA209) and possesses several endocrinological features, including the expression of progesterone receptor, activin type II, activin βA and activin βB. In addition, it also retains the ability to respond to cAMP stimulation and secrete PRL-related hormones, a hallmark of the antimesometrial decidual cells. This cell line is useful for studying the mechanism of decidual cell regression and reorganization.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow II medium available from ABM (TM002). To make the complete growth medium, add the following components to the base medium: 10% fetal bovine serum (TM999) and Penicillin/streptomycin. Atmosphere: air: 95%, CO2: 5%; Temperature: 33.0°C. The functional expression of the SV40 antigen is induced by culturing the cells in vitro at a temperature permissive (33°C). At a non-permissive temperature (37°C-39°C), the cells cease to proliferate and revert to differentiated phenotype.
Preservation	1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control	1) Northern blot performed to analyze presence of activin βA , follistatin and PLP-B mRNA; 2) RT-PCR analysis of progesterone receptor, activin βA, activin βB, and activin receptor II mRNA was completed with L19 ribosomal protein mRNA as control.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.