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Immortalized-Mouse-Cementoblasts

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Immortalized-Mouse-Cementoblasts

商品詳情參考文獻相關資料

	Print Version
BioSafety Level	II
Organism	CD-1 Mouse
Source Organ	Mandibular First Molars
Growth Properties	Adherent
Morphology	Polygonal
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	Bone sialoprotein (BSP), Osteocalcin (OC), Osteopontin (OPN), Type I collagen
Applications	For Research Use Only
Immortalization Method	Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
Description	The cementum is a mineralized tissue lining the tooth root surface and shares many properties with mineralized tissues such as the bone. It has a role in reparative activities, the regeneration of tissues, and is responsible in maintaining healthy periodontium. The Immortalized Mouse Cementoblasts is immortalized with mouse SV40 large T antigen, and retains phenotypic characteristics of the primary cells such as the expression of parathyroid hormone 1 receptor (PTH-1), noncollagenous proteins (BSP, OC, OPN), and type I collagen. Furthermore, this cell line has been shown to respond to the presence of the parathyroid hormone (PTH) and the parathyroid hormone-related protein (PTHrP), and is able to stimulate mineral nodule formation, which makes it a useful tool to study the mechanisms involved in regulating the molecular biological activity and properties of the cementum or mineralized tissues, biomineralization, and affects of the PTH-1 receptor.
Procedure Overview
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Propagation	The base medium for this cell line is Prigrow III medium available from abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Preservation	1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control	Expression of PTH-1 was assessed by Northern blot and gene-expression assay. Markers were analyzed by RT-PCR.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.