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Human-Endometrial-Somatic-Stem-Cells-(Stromal-Fraction-hESP-ICEs)

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Human-Endometrial-Somatic-Stem-Cells-(Stromal-Fraction-hESP-ICEs)

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Human-Endometrial-Somatic-Stem-Cells-(Stromal-Fraction-hESP-ICEs)

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Description	A specialized somatic stem cell (SSC) population in the human endometrium, often identified by using the Side Population (SP) technique, is thought to mediate the endometrial regeneration. The Human Endometrial Somatic Stem Cells are established from human endometrial side population cells, specifically from the Epithelial fraction (ICEp; Cat. No. T4107) or the Stromal fraction (ICEs; Cat. No. T4108). Both cell types retain the ability to undergo osteogenic and adipogenic differentiation in vitro . After adipogenic induction, the cells show lipid accumulation in the cytoplasm and increased expression of lipoprotein lipase (LPL). When under osteogenic induction, the cells stain positive for bonesialoprotein (BSP) and show increased expression of osterix. Furthermore, both cell types are capable of reconstructing total endometrial-like tissue in vivo when transplanted beneath the renal capsule of NOD-SCID mice. The Human Endometrial Somatic Stem Cells serve as a reliable model to test relevant targets for endometrial physiology and pathology.
Procedure Overview
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Human endometrium from healthy women
Isolation	Endometrial Somatic Stem Cells are isolated based on the Side Population (SP) technique- a method that replies on the capability of cells to exclude the DNA binding Hoechst 33342 dye. Cells are labeled and sorted by flow cytometry based on low Hoechst 333.
Growth Properties	Adherent
Morphology	Fibroblast-like
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Population Doubling	Proliferation ceases after about 20 weeks of culture.
Markers	1) Purity and origin of cells are confirmed by expression of CD9 (epithelial marker) or vimentin (stromal marker) and CD90, CD73 and CD105 (mesenchymal attributes); 2) Undifferentiated status of the cells are confirmed by expression of OCT4, NANOG, GDF3.
Applications	For Research Use Only
Disease	Normal
Propagation	Use of PriCoat? T25 Flasks (G299) or . Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow IV medium available in ABM, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10%, L-glutamine to 2mM and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO₂), 5%.
Reference	1) Cervelló, I., et al (2010). Human Endometrial Side Population Cells Exhibit Genotypic, Phenotypic and Functional Features of Somatic Stem Cells. PLoS One 5 (6): e10694. 2) Cervelló, I., et al (2011).
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.