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AZ-AHR Stable HepG2 Luciferase Reporter Cell Line

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AZ-AHR Stable HepG2 Luciferase Reporter Cell Line

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AZ-AHR Stable HepG2 Luciferase Reporter Cell Line

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	Print Version
BioSafety Level	II
Organism	Homo sapiens
SourceOrgan	Liver
Growth Properties	Adherent
Morphology	Epithelial-like
Description	The Aryl hydrocarbon receptor (AhR) is a ligand-activated transcriptional factor that regulates genes implicated in drug metabolism, cell proliferation, immune responses, circadian rhythm and tumor promotion. Due to the numerous and varied effects that AhR activation may have on the cell, research investigating novel AhR ligands is important for drug interactions and chemically induced carcinogenesis. The AZ-AHR Stable HepG2 Luciferase Reporter Cell Line is a sophisticated and highly sensitive tool for gene reporter assays through the dose- and time-dependent induction of luciferase activity in response to various AhR ligands. This immortalized cell line also remains fully functional for over 15 passages increasing efficiency and consistency between experiments.
Procedure Overview
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available in ABM, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, 4 mM L-glutamine, 1% nonessential amino acids, 1mM sodium pyruvate and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	Selective growth on media containing hygromycin
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.