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GRPR-Luciferase Stable PC3 Cell Line

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GRPR-Luciferase Stable PC3 Cell Line

商品詳情參考文獻(xiàn) 相關(guān)資料

	Print Version
BioSafety Level	II
Organism	Homo sapiens
SourceOrgan	prostate
Growth Properties	Adherent
Morphology	epithelial
Description	The PC3 prostate cancer cell line is widely used to represent a highly aggressive form of prostate cancer. The GRPR-PC3-Luciferase Stable Cell Line has been stably transfected with pcDNA3.1/hygro/Luc705, resulting in the insertion of the Luc705 cassette. The Luc705 cassette is a Firefly Luciferase coding sequence containing an aberrant intron; which prevents the correct expression of the Luciferase gene. Removal of the aberrant by various mechanisms - such as a splice shifting oligonucleotide (Oligonucleotide Treatment) - allows for proper translation of the gene, resulting in bioluminescence of the cell. The GRPR-PC3-Luciferase Stable Cell Line is useful for studying intracellular delivery of oligonucleotides and oligonucleotdie-conjugates via receptor mediated targeting.
Procedure Overview
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow IV medium available in ABM, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO₂), 5%.
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Quality Control	An oligonucleotide treatment was used to validate the GRPR-PC3-Luciferase Stable cell line. Cells post-treatment was tested for 1) Luciferase activity/bioluminescence using luciferase assay and 2) Expression level of correctly transcribed luciferase gene using RT-PCR.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.