丁香五月激情五月激情五月,免费在线观看一区,丁香五月四房播播

您好，歡迎來到拜力生物網(wǎng)站！請(qǐng)登錄注冊(cè)

我的訂單 | 聯(lián)系我們 | 在線客服QQ：3012681483 | 客服電話：400-968-7988 | 工作時(shí)間：08:30-17:30

主營(yíng)產(chǎn)品： ELISA試劑盒、抗體、補(bǔ)體血清、兔補(bǔ)體、干擾素、小鼠測(cè)溫儀、進(jìn)口試劑、Quidel代理、Swant代理、PBL Assay Science代理

網(wǎng)站首頁(yè) 產(chǎn)品中心代理品牌新聞資訊資源中心關(guān)于我們

所在位置：首頁(yè) > 產(chǎn)品中心 > abmgood > abmgood產(chǎn)品

訂購(gòu)信息

上海拜力生物科技公司

Tel:400-968-7988 021-33779008

iPS-27 Cells

品牌：Abmgood

貨號(hào)：

規(guī)格：

貨期：

應(yīng)用：

咨詢客服

立即購(gòu)買加入購(gòu)物車

iPS-27 Cells

商品詳情參考文獻(xiàn) 相關(guān)資料

	Print Version
Safety	BioSafety Level II
Organism	Human
Source Organ	Fetal Lung Fibroblasts (MRC-5)
Picture
Image
Concentration	10⁶ Cells/ml
Induction	iPS-27 was generated using lentiviral transduction of human fetal lung fibroblasts with cMYC, KLF4, OCT4 and SOX2.
Pluripotency	Positive for markers Nanog, Tra-1-60, Tra-1-80 and SSEA-3 by immunofluorescence and flow cytometry. Capable of differentiating into derivatives of the 3 germ layers.
Propagation	The base medium for this cell line is Prigrow IV medium available from ABM (TM004). To make the complete growth medium, add the following components to the base medium: serum replacer to a final concentration of 20%; b-FGF to a final concentration of 6ng/ml; 1 mM L-glutamine and 2-mercaptoethanol solution; 1X Non-essential amino acids. Sterilize the media through a 0.22 micron filter before use. This cell line needs to be cultured on mouse embryonic fibroblasts (ABM T2001) feeder cells. Atmosphere:air: 95%, CO₂: 5%; Temperature: 37.0°C.
Subculturing	1. Remove and discard culture medium. 2. Add 2.0mL of Dispase solution to flask and incubate the cells at 37°C to facilitate dispersal. Observe cells under an inverted microscope until cell layer is dispersed. 3.Transfer the dispase solutions containing colonies into a conical tube. 4. Centrifuge cells at 1000rpm for 3 minutes to pellet. 5. Gently aspirate out the solution, leaving pellet undisturbed. 6. Resuspend pellet in fresh culture medium and plate on MEF feeder plates. 7. Incubate cultures at 37°C.
Storage	-180°C
Shipping	On Dry Ice
Notes	For Research Use Only, not for therapeutic or diagnostic purposes.
References	1. Stuart M Chambers, Christopher A Fasano, Eirni P Papapetrou, Mark Tomishima, Michel Sadelain and Lorenz Studer. Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol. 26, 275-280 (2009). 2. Papapetrou EP, Tomishima MJ, Chambers SM, Mica Y, Reed E, Menon J, Tabar V, Mo Q, Studer L, Sadelain M. Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation. Proc Natl Acad Sci U S A. Aug 4;106(31):12759-64. (2009).