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Human Primary Dopaminergic Neuronal Precursor Cell

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Human Primary Dopaminergic Neuronal Precursor Cell

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Human Primary Dopaminergic Neuronal Precursor Cell

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	Print Version
Procedure Overview
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Brain
Isolation	Selected in tyrosine free medium supplemented with a mixture of growth factors
Growth Properties	Adherent
Morphology	Multipolar
Image
Image
Passage Number	2
Markers	tyrosine hydroxylase (TH)
Applications	For Research Use Only
Donor Age	Fetal Tissue - 14-16 weeks gestation
Donor Gender	undetermined
Disease	Normal
Propagation	Use of PriCoat? T25 Flasks (G299) or . Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is PriGrow IV medium available in ABM, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 5%, bFGF and GDNF to 10ng/ml and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO₂), 5%. To differentiate the cells into neurons, plate the cells on poly lysine (PLL) coated plates at a density of 10⁴ cells per cm², in PriGrow IV containing 5% fetal bovine serum, 10ng/ml each of bFGF and human EGF, and 100uM dibuturyl cAMP. The cells should be left in the differentiation medium for at least one week before testing for neuron specific markers.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.