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Pat5 Embryonic Stem Cells

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Pat5 Embryonic Stem Cells

商品詳情參考文獻(xiàn) 相關(guān)資料

	Print Version
Safety	BioSafety Level II
Organism	129X1/SvJ mouse
Source Organ	Embryo
Description	Pat5 Embryonic Stem Cells were derived from 129X1/SvJ mouse embryos and can be utilized to introduce genetic mutations with gene targeting vectors through homologous recombination. Growth Properties: Adherent Morphology: Polygonal
Concentration	10⁶cells/vial
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. Cell line must be maintained on γ-irradiated mouse embryonic fibroblasts (T2003). The base medium for this cell line is Prigrow III medium available in abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10% and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Storage	-180°C
Shipping	On Dry Ice
Notes	For Research Use Only, not for therapeutic or diagnostic purposes.