免费网站观看WWW免费下载,五月色婷婷亚洲男人的天堂

您好，歡迎來到拜力生物網站！請登錄注冊

我的訂單 | 聯(lián)系我們 | 在線客服QQ：3012681483 | 客服電話：400-968-7988 | 工作時間：08:30-17:30

主營產品： ELISA試劑盒、抗體、補體血清、兔補體、干擾素、小鼠測溫儀、進口試劑、Quidel代理、Swant代理、PBL Assay Science代理

網站首頁產品中心代理品牌新聞資訊資源中心關于我們

所在位置：首頁 > 產品中心 > abmgood > abmgood產品

Immortalized-Rat-Retinal-Capillary-Endothelial-Cells-SV40

訂購信息

上海拜力生物科技公司

Tel:400-968-7988 021-33779008

Immortalized-Rat-Retinal-Capillary-Endothelial-Cells-SV40

品牌：Abmgood

貨號：

規(guī)格：

貨期：

應用：

咨詢客服

立即購買加入購物車

Immortalized-Rat-Retinal-Capillary-Endothelial-Cells-SV40

商品詳情參考文獻相關資料

	Print Version
BioSafety Level	II
Organism	Rattus norvegicus
Source Organ	Eye
Growth Properties	Adherent
Morphology	Polygonal
Passage Number	P10
Population Doubling	24-28 hours
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Immortalization Method	Serial passaging and infection with Lentivirus carrying SV40 antigen gene
Description	The Immortalized Rat Retinal Capillary Endothelial Cells are immortalized with SV40 antigen. These cells are useful in studying the transport mechanism of molecules across the inner blood-retinal barrier (inner BRB).
Procedure Overview
Image
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow IV medium available in ABM, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0°C
Subculturing	1. Remove and discard culture medium. 2. Add 2.0mL of Trypsin-EDTA (TM050) solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 3. Centrifuge cells at 1500rpm for 3 minutes to pellet. 4. Aspirate out trypsin, leaving pellet undisturbed. 5. Resuspend pellet in fresh culture medium and plate in new culture vessel. 6. Incubate cultures at 37°C. Be sure to use T25 ECM-coated flasks(G299) for growth.
Preservation	1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control	Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.