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Immortalized-Human-Ascending-Aorta-Vascular-Smooth-Muscle-Cells

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Immortalized-Human-Ascending-Aorta-Vascular-Smooth-Muscle-Cells

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Immortalized-Human-Ascending-Aorta-Vascular-Smooth-Muscle-Cells

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	Print Version
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Heart
Donor Age	46
Donor Gender	Male
Growth Properties	Adherent
Morphology	Polygonal (varies depending on phenotype)
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Immortalization Method	Transfection with mammalian expression vector carrying the large T SV40 antigen
Description	Vascular Smooth Muscle Cells (VSMCs) can perform contractile as well as synthetic functions which are associated with changes in morphology, proliferation rates, and the expression of different marker proteins such as TGFβ. Immortalized Human Ascending Aorta Vascular Smooth Muscle Cells are isolated from a male patient with bicuspid aortic valve (BAV). The phenotype varies from quiescent and contractile to proliferative and synthetic which can perform an array of functions depending on the phenotype. The immortalized cells are valuable for research studies of the expression and regulation of genes particularly endoglin. Endoglin is downregulated in the Immortalized Human Ascending Aorta Vascular Smooth Muscle Cells in response to treatment with the drug Losartan (Cozaar) in various concentrations.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is PriGrow I medium available from abm (TM001). To make the completed growth medium, add the following components to the base medium: Fetal Bovine Serum (TM999) to final concentration of 20% and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	1) Spectral karyotyping (SKY) used for chromosome analysis, 2) Immunofluorescence used to determine presence of large T SV40 antigen, and 3) Flow cytometry used for characterization.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.