欧美性生交XXXXX无码十全,婷婷免费伊人,婷婷五月在线视频

您好，歡迎來(lái)到拜力生物網(wǎng)站！請(qǐng)登錄注冊(cè)

我的訂單 | 聯(lián)系我們 | 在線客服QQ：3012681483 | 客服電話：400-968-7988 | 工作時(shí)間：08:30-17:30

主營(yíng)產(chǎn)品： ELISA試劑盒、抗體、補(bǔ)體血清、兔補(bǔ)體、干擾素、小鼠測(cè)溫儀、進(jìn)口試劑、Quidel代理、Swant代理、PBL Assay Science代理

網(wǎng)站首頁(yè) 產(chǎn)品中心代理品牌新聞資訊資源中心關(guān)于我們

所在位置：首頁(yè) > 產(chǎn)品中心 > abmgood > abmgood產(chǎn)品

Immortalized-Mouse-Enteric-Neuronal-Cells-

訂購(gòu)信息

上海拜力生物科技公司

Tel:400-968-7988 021-33779008

Immortalized-Mouse-Enteric-Neuronal-Cells-

品牌：Abmgood

貨號(hào)：

規(guī)格：

貨期：

應(yīng)用：

咨詢客服

立即購(gòu)買(mǎi) 加入購(gòu)物車(chē)

Immortalized-Mouse-Enteric-Neuronal-Cells-

商品詳情參考文獻(xiàn) 相關(guān)資料

	Print Version
BioSafety Level	II
Organism	H-2Kb-tsA58 Transgenic Mice
Source Organ	Intestine of E13 Mouse Fetus
Growth Properties	Adherent
Morphology	Bipolar
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	Peripherin, HuD, PGP9.5, tau, synaptophysin, characteristic enteric neuron receptors, cRet, nestin
Applications	For Research Use Only
Immortalization Method	Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
Description	Immortalized Mouse Enteric Neuronal Cells were generated by transfecting mouse intestinal cells with the SV40 large T antigen. These novel cells are very stable- biologically and phenotypically- for multiple generations, in vitro. Thus, they are perfectly suited for research focusing on the enteric nervous system; from determining the impact of disease on the enteric nervous system to simply tracking its development.
Procedure Overview
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is PriGrow IV available from abm (TM004). To make the complete growth medium, add the following components to the base medium: 520 ng/ml selenium (Sigma), 16.11 μg/ml putrescine (Sigma), 5 μg/ml insulin (Sigma), 1 μg/ml transferring (Sigma), 0.1 mg/ml fetuin (Sigma), 1 μg/ml BSA (Bio Basic), and 6.3 ng/ml progesterone (Sigma). Filter the media with 0.45 μM filter before proceeding to add a final concentration of 10% fetal bovine serum (TM999), 1% Penicillin/Streptomycin (G255), 20 U/mL mouse IFN-gamma (Z200085), and 100 ng/mL glial cell line-derived neurotrophic factor (GDNF) (Z101059) to make complete growth media. Culture cells under the following conditions: Atmosphere: air: 90%, CO?: 10%; Temperature: 33.0°C.
Quality Control	Neuronal markers were assessed and confirmed via western blot and immunocytochemical analysis.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.