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Immortalized-Rat-Odontoblast-Cells

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Immortalized-Rat-Odontoblast-Cells

商品詳情參考文獻相關資料

	Print Version
BioSafety Level	II
Organism	3-5 day old Sprague-Dawley Rats
Source Organ	Molar tooth germs
Growth Properties	Adherent
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	DMP1, DMP2, DMP3
Applications	For Research Use Only
Immortalization Method	Human telomerase reverse transcriptase (pCI-Neo-hTERT)
Description	Odontoblasts are useful in studying the development of mineralized tissue, such as dentin. They are especially useful if performing experiments relating to characterization of odontoblast-specific genes in dentinogenesis. The Immortalized Rat Odontoblast Cells retain the expression of proteins involved in dentinogenesis, namely the dentin matrix proteins DMP1, DMP2, and DMP3, which are needed for development of mineralized dentin. Other proteins which are expressed by this cell line are the extracellular matrix proteins alkaline phosphatise (ALP), CBFA1, collagen type 1 (COL1), and amenoblastin (AMBN). This cell line is also capable of mineralization in in vitro and in vivo settings, making them ideal for studying dentin formation, genes implicated in odontoblast development and differentiation, as well as tissue engineering to make reparative dentin.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow IV medium available from ABM (TM004). To make the completed growth medium, add the following components to the base medium: 15% fetal bovine serum (TM999), 50μg/mL ascorbic acid and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Preservation	1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control	(1) Expression of DMP1, DMP2, and DMP3 detected by RT-PCR and immunostaining (2) in vitro mineralization activity confirmed by Von Kossa staining; (3) in vivo mineralization activity verified bysubcutaneous and intramuscular implantation.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.