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$Immortalized-Human-Hair-Follicle-Dermal-Papilla-Cells-\u2013SV40T\/c-Myc--$

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Immortalized-Human-Hair-Follicle-Dermal-Papilla-Cells-\u2013SV40T\/c-Myc--

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Immortalized-Human-Hair-Follicle-Dermal-Papilla-Cells-\u2013SV40T\/c-Myc--

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	Print Version
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Hair follicles
Growth Properties	Adherent
Morphology	flat, polygonal
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	smooth muscle alpha actin, alkaline phosphatase
Applications	For Research Use Only
Immortalization Method	Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen and c-Myc gene
Description	Human Hair Follicle Dermal Papilla cells are highly active mesenchymal cells isolated from the hair papilla embedded in extracellular matrix of scalp hair follicles. Dermal Papilla Cells play a significant role in controlling the hair growth cycle and production by involving in the epithelial-mesenchymal interaction of hair follicle cells. The Immortalized Human Dermal Papilla Cells (DPCs) express DPC specific markers smooth muscle alpha actin and alkaline phosphatase and retain the ability to secrete growth factors such as VEGF, PIGF, bFGF, IGFBP-6 and GM-CSF. DCP-conditioned media have also been shown to increase proliferation of HaCaT cells in vitro and to accelerate hair growth in mice models. A list of up-regulated genes in the DPC can be found in the supporting document section below. The DPC serves as a useful tool for the study of hair growth regulation.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from ABM (TM003). To make the complete growth medium, add the following components to the base medium: 10% fetal bovine serum (TM999) and Penicillin/streptomycin. Atmosphere: air: 95%, CO2: 5%; Temperature: 37.0°C.
Preservation	1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control	1) Western blot was used to identify SV40T and c-myc transgene expression. 2) Immunocytochemistry and western blot were performed to assess the expression of smooth muscle actin alpha and alkaline phosphatase. 3) Up-regulated genes in DPCs is analyzed by DAVID (the Database for Annotation, Visualization and Integrated Discovery).
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.