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Human-Normal-Bladder-Smooth-Muscle-Cells

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Human-Normal-Bladder-Smooth-Muscle-Cells

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Human-Normal-Bladder-Smooth-Muscle-Cells

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	Print Version
Description	abm’s Human Urinary Bladder Smooth Muscle Cells maintain the expression of smooth muscle myosin and alpha-actin, and most importantly, these cells retained their ability to contract in response to a physiological agonist. This contractile ability can be useful in testing the effectiveness of pharmacologic agents on smooth muscle contractions. Another unique feature of this cell line is its expression of myosin isoform SM-B, which can be used for studies involving alternative splicing at the 3’end of the SM myosin heavy chain gene.
Procedure Overview
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Urinary Bladder
Growth Properties	Adherent
Morphology	Elongated with tapered ends, and in high density cultures, they form swirls of cells arranged in parallel
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Markers	Myosin isoforms (N-terminal isoforms SM-A/B and C-terminal isoforms SM1/2), SM22, alpha-smooth muscle actin, h-caldesmon, Ca+2-dependent myosin light chain kinase, and protein kinase G
Applications	For research only. The Human Urinary Bladder Smooth Muscle Cell is unique in its 1. Ability to contract upon exposure to 10uM bethanechol and the contraction is reversible by washing away the drug; 2. First known established smooth muscle cell line to ex相?盹??盷?腱盼????帉盺??
Donor Gender	Female
Disease	Normal
Propagation	Use of PriCoat? T25 Flasks (G299) or . Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow I medium available in ABM, Cat. No. TM001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO₂), 5%.
Quality Control	Immunofluorescence, RT-PCR and Western Blot were used to confirm the presence of Smooth Muscle Markers such as alpha-SM actin, N-and C- terminal MHC isoforms (SM-A, SM-B, SM1 and SM2), SM22, and h-caldesmon.
Reference	Chacko, S., et al. (2012). Generation of a human urinary bladder smooth muscle cell line. In Vitro Cell Dev Biol Anim 48(2):84-96. PMID:22259013
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.