Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells.
Subtype
polyclonal
Application
WB , ELISA , IF
Raised In
Rabbit
Immunogen
Fusion protein to NANOG with GST tag purified from E.coli
Guarantee
abm guarantees that all our Anti-NanogRabbit Polyclonal Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Properties
Formulation
Liquid
Quantity
100 μl
Clone
Polyclonal
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Buffer
PBS, pH 7.4 with 0.02% sodium azide.
Concentration
100μg/100ul
Purity
Affinity purified
Isotype
IgG
Species Reactivity
human
Anti-NanogRabbit Polyclonal Antibody Images
Specific References
Y030044 has not yet been referenced specifically in any publications.