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Thomson Factor Polycistronic Adenovirus (Human)

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Thomson Factor Polycistronic Adenovirus (Human)

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Thomson Factor Polycistronic Adenovirus (Human)

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	Print Version
Vector	pAdeno
Promoter	CMV
Insert	Thomson Factor Polycistronic
Picture
Organism	Human
Accession #	Refer to individual adenovirus
Alias	Oct4, Sox2, Nanog and Lin28
Functions	Thomson iPSC factors (Oct4, Sox2, Nanog and Lin28) expressed from a single CMV promoter in a single adenoviral vector. All four factors are linked by protein 2A sequence which allows similar levels of transgene expression of four factors in target cells, thus streamlining iPSC reprogramming. A GFP control adenovirus is included for easy tracking of gene transduction efficiency.
Gene Type	cDNA
Purification	By Ion-Exchange column
Quality Control	By sequencing and Western blot analysis of transgene expression in transduced cells. Functional test includes iPSC reprogramming.
Titer	>1x10⁶pfu/mL
Storage	10mM Tris-HCl, pH 8.0. 2.5% glycerol
Shipping	High titer adenoviruses are shipped with dry ice and low titer adenoviruses are shipped with ice packs. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles.
Notes	For Research Use Only, not for therapeutic or diagnostic purposes.
References	Induced pluripotent stem cell lines derived from human somatic cells. Yu et al. Science. 2007 Dec 21;318(5858):1917-20. Epub 2007 Nov 20. Human induced pluripotent stem cells free of vector and transgene sequences. Yu et al. Science. 2009 May 8;324(5928):797-801. Epub 2009 Mar 26. Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Kaji et al. Nature. 2009 Apr 9;458(7239):771-5. Epub 2009 Mar 1.