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Immortalized-Human-Podocytes

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Immortalized-Human-Podocytes

商品詳情參考文獻(xiàn) 相關(guān)資料

	Print Version
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Urinary Tissue
Donor Gender	Male
Growth Properties	Adherent
Morphology	Cuboidal
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Immortalization Method	Co-infected with retrovirus carrying the thermosensitive SV40 large T antigen (U19tsA58) and with retrovirus carrying human telomerase (hTERT)
Description	Immortalized Human Podocytes are podocytes isolated from urine samples from healthy donor, and had been transformation with the thermosensitive SV40 large T antigen (U19tsA58) together with human telomerase (hTERT). The cells retained their primary cell characteristics with podocyte markers WT1, synaptopodin, and nestin. The urinary podocytes can be cultured for long-term and is a valuable tool for studies relating to pathogenesis and mechanisms of podocyte injury.
Procedure Overview
Image
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow II medium available from abm (TM002). To make the completed growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum (TM999) to final concentration of 10%, 1x insulin-transferrin-selenium G supplement and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 33.0°C.
Quality Control	1) Cultures were selected with 0.2 mg/ml G418 for T antigen, and 0.05 mg/ml hygromycin for hTERT; 2) Western blot preformed to determine SV40 T antigen expression
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.