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Immortalized-Mouse-Schwann-Cells

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Immortalized-Mouse-Schwann-Cells

商品詳情參考文獻相關(guān)資料

	Print Version
BioSafety Level	II
Organism	(twi/+) Mice
Source Organ	Brain
Growth Properties	Adherent
Morphology	Spindle-shaped
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Immortalization Method	Spontaneous immortalization
Description	Immortalized Mouse Schwann Cells are derived from Twitcher mice. The Schwann cells were immortalized spontaneously in culture, dubbed as the TwS1 cells, and can be used as a murine model for globoid cell leukodystrophy (Krabbe disease) studies. Unlike the normal mouse Schwann cells (Immortalized Mouse Schwann Cells (IMS32), T0295), the TwS1 cells have a nonsense mutation in the GALC genome which is associated with the pathogenesis of Krabbe disease. These cells also exhibit markers for S100, laminin, p75 ^NTR, GFAP and vimentin.
Procedure Overview
Image
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10% and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C. The growth of TwS1 cells is cell density-dependent. The cells should be seeded in rather high density, otherwise their growth becomes very slow.
Preservation	1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control	Immunofluorescence detection for S100, laminin, and p75 ^NTR, and for GFAP and vimentin markers
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.