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$Immortalized-Mouse-Smooth-Muscle\/Myometrial-Like-Cells-(SMU1-10)$

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Immortalized-Mouse-Smooth-Muscle\/Myometrial-Like-Cells-(SMU1-10)

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Immortalized-Mouse-Smooth-Muscle\/Myometrial-Like-Cells-(SMU1-10)

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	Print Version
BioSafety Level	II
Organism	H-2K^b-tsA58 transgenic Mouse
Source Organ	Uterus
Donor Gender	Female
Growth Properties	Adherent
Morphology	Polygonal
Population Doubling	26 hours
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	SM-MHC, SM22 alpha, connexin 43, thromboxane A2 receptor, progesterone receptor
Applications	For Research Use Only
Immortalization Method	Isolated from a H-2Kb-tsA58 transgenic mouse carrying a thermolabile SV40 large T-antigen gene
Description	During preganancy the myometrium undergoes hypertrophy and hyperplasia. The established Immortalized (Conditionally) Mouse Smooth Muscle/Myometrial-Like Cells (SMU1-10) is recommended for use as an in vitro model of smooth muscle/myometrial differention and for steroid hormone gene regulation studies. It proliferates at a permissive temperature of 33°C and exhibits smooth-muscle related markers such as the SM-MHC, SM22 alpha, and connexin 43. Thromboxane A2 receptors and progesterone receptors are also present. The SMU1-10 cells expresses smooth muscle gamma-actin (SMGA) which is a maker for smooth muscle differentiation. This cell line is recommended for studies and analysis in smooth muscle/myometrial differentiation, maturation, and the role of steroid hormones in gene regulation.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from ABM (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, 10 U/ml IFN-γ, and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 33.0°C.
Quality Control	Indirect immunofluorescence, Northern blot, and RT-PCR were utilized for smooth muscle-related markers
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.