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Immortalized-Mouse-Hepatic-Sinusoidal-Endothelial-Cells---SV40

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Immortalized-Mouse-Hepatic-Sinusoidal-Endothelial-Cells---SV40

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Immortalized-Mouse-Hepatic-Sinusoidal-Endothelial-Cells---SV40

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	Print Version
BioSafety Level	II
Organism	Mus musculus
Source Organ	Liver
Growth Properties	Adherent
Morphology	Cobblestone-like
Population Doubling	population doubling time is 10 hours
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	vWF and caveolin-1
Applications	For research only. The Immortalized Mouse Hepatic Sinusoidal Endothelial Cells- SV40 is unique in its 1. Ability to endocytose AcLDL; 2. Increased migration rate to VEGF and FGF; 3. Ability to form robust vascular tube-like structures; 4. 90% transduction efficiency using retrovirus and 5. Tolerance to serum starvation.
Immortalization Method	Serial passaging and transduction with pantropic recombinant retroviruses carrying SV40 Large T antigen
Cell Type Characterization
Description	Hepatic sinusoidal endothelial cells (HSECs) are an unique sub-population of liver that lines the hepatic sinusoids. Their role involves in clearing waste products from the blood, regulating pericyte contractility and innate immune functions. The immortalized Mouse Hepatic Sinusoidal Endothelial Cells- SV40 is derived from murine HSECs that have maintained endothelial characteristics and HSEC-specific features such as vWF and caveolin-1. These cells also migrate in response to angiogenici growth factors, form vascular tube-like structures, endycytose acetylated low-density lipoprotein (AcLDL) and secrete proteins involved in matrix remodelling. In addition, these cells are easily transduced by retrovirus. The Immortalized Mouse Hepatic Sinusoidal Endothelial Cells- SV40 is therefore suitable for studying liver endothelial cell biology, including motility and angiogenesis.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow I medium available from ABM (TM001). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 5%, Penicillin/Streptomycin(G255). Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C.
Quality Control	1. Real Time PCR was used to quantify SV40 gene expression in immortalized cell line. 2. Western blot was used to confirm the presence of endothelial markers such as vWF and caveolin-1.
Reference	Huebert, R.C., et al (2010). Immortalized liver endothelial cells: a cell culture model for studies of motility and angiogenesis. Laboratory Investigation (2010) 90, 1770–1781.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.