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Human-Normal-Subcutaneous-Adipocytes

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Human-Normal-Subcutaneous-Adipocytes

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	Print Version
Description	Generally mature adipocytes isolated from Human tissue cannot survive 24 hours after isolation. In order to facilitate research in cell energy storage and metabolic homeostasis, abm provides mature adipocytes differentiated from pre-adipocytes. These mature adipocytes are shipped as live cells in a T25 flask 7-10 days after induction of differentiation and small oil droplets will be visible and will continue to accumulate into big locules. Upon cell arrival, it is recommended to use the adipocytes within 3 weeks.
Procedure Overview
BioSafety Level	II
Organism	Homo sapiens
Source Organ	subcutaneous adipose tissue
Growth Properties	Adherent
Morphology	Polygonal
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Passage Number	2
Recommended Seeding Density	10,000-15,000 cells/cm²
Markers	oil red o staining, nile red staining of lamellar bodies
Applications	For Research Use Only
Donor Age	42
Donor Gender	Male, BMI = 24.62
Donor Ethnicity	Caucasian
Disease	Normal
Propagation	The basal medium for this cell line is Prigrow IV medium available in abm, Cat. No. TM004. To make the complete growth medium, add the following components to the basal medium: 10% fetal bovine serum (TM999), 8 ug/ml d-biotin, 0.5 ug/ml human insulin, 400 ng/ml dexamethasone and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO₂), 5%.
Subculturing	Human Normal Subcutaneous Adipocytes can be split into different culture vessels (i.e. multi-well plates) for experimental needs, but differentiated adipocytes will not proliferate in vitro.
Preservation	Human Normal Subcutaneous Adipocytes cannot be cryopreserved.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.