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Human-Normal-Synovial-Membrane-Fibroblasts

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Human-Normal-Synovial-Membrane-Fibroblasts

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Human-Normal-Synovial-Membrane-Fibroblasts

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Description	Synovial membrane fibroblasts are predominant cells isolated in synovial membrane which maintain joint homeostasis by secreting synovial fluid components for normal blood/synovial fluid exchanges. Synovial fibroblasts also promote inflammation and contribute to cartilage destruction by secreting effector molecules such as cytokines and metalloproteinases. These cells are known to play a major role in the pathogenesis of rheumatoid arthritis.
Procedure Overview
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Synovial Membrane
Growth Properties	Adherent
Morphology	Multipolar
Passage Number	2
Population Doubling	6 PD. Doubling time: 84.4 hours
Recommended Seeding Density	7,000 - 10,000 cells per cm²
Applications	For Research Use Only
Donor Age	40
Donor Gender	Female
Donor Ethnicity	Caucasian
Disease	Normal
Propagation	Use of PriCoat? T25 Flasks (G299) or . Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available in abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10%, 1% L-glutamine (2mM) and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO₂), 5%.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.