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Human-Primary-Peripheral-Blood-Mononuclear-Cells

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Human-Primary-Peripheral-Blood-Mononuclear-Cells

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Human-Primary-Peripheral-Blood-Mononuclear-Cells

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Description	Human Peripheral Blood Mononuclear Cells (PBMC) mainly consists of lymphocytes and monocytes, which are involved in various defense mechanisms in the immune system. The PBMCs secret cytokines and chemokines to regulate immune response as well as inflammation in the human body. These cells are useful in the fields of immunology, infectious disease, hematological malignancies, vaccine development and transplant immunology.
Procedure Overview
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Blood
Isolation	These cells are extracted from buffy coats using Lymphoprep(R), a hydrophilic polysaccharide that separates layers of blood, with monocytes and lymphocytes forming a buffy coat under a layer of plasma. This buffy coat contains the PBMCs.
Growth Properties	Suspension
Morphology	Spheric
Passage Number	The PBMCs are cryopreserved immediately after isolation
Population Doubling	The PMBCs do not proliferate in vitro
Applications	For Research Use Only
Disease	Normal
Propagation	The basal medium for this cell line is Prigrow X series medium available in ABM, Cat. No. TM4103. Atmosphere: air, 95%; Carbon dioxide (CO₂), 5%. The Human PBMCs do not proliferate in vitro .
Quality Control	abm’s cryopreserved PBMCs consistently exhibited viability above 87% (Day 0 when thawed) with less than a 7.5% deviation from that of fresh PBMCs.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.