最近中文字幕MV2018在线高清,欧美亚洲久久综合,综合图区网友自拍

您好，歡迎來到拜力生物網(wǎng)站！請登錄注冊

我的訂單 | 聯(lián)系我們 | 在線客服QQ：3012681483 | 客服電話：400-968-7988 | 工作時(shí)間：08:30-17:30

主營產(chǎn)品： ELISA試劑盒、抗體、補(bǔ)體血清、兔補(bǔ)體、干擾素、小鼠測溫儀、進(jìn)口試劑、Quidel代理、Swant代理、PBL Assay Science代理

網(wǎng)站首頁產(chǎn)品中心代理品牌新聞資訊資源中心關(guān)于我們

所在位置：首頁 > 產(chǎn)品中心 > abmgood > abmgood產(chǎn)品

HEK293S-TetR GnTI- Stably Expressing TP Cell Line

訂購信息

上海拜力生物科技公司

Tel:400-968-7988 021-33779008

HEK293S-TetR GnTI- Stably Expressing TP Cell Line

品牌：Abmgood

貨號：

規(guī)格：

貨期：

應(yīng)用：

咨詢客服

立即購買加入購物車

HEK293S-TetR GnTI- Stably Expressing TP Cell Line

商品詳情參考文獻(xiàn) 相關(guān)資料

	Print Version
BioSafety Level	II
Organism	Homo sapiens
SourceOrgan	Kidney
Growth Properties	Adherent
Morphology	Polygonal
Description	HEK293S-TetR GnTI- Stably Expressing TP Cell Line was established to have a tetracycline-inducible HEK293S stable cell line which expresses the thromboxane A2 receptor (TP). TP is a G-protein coupled receptor (GPCR) involved in vasoconstriction and thrombosis. HEK293S-TetR GnTI- cells do not express the N-acetylglucosaminyltransferase I (GnTI). It is valuable tool for discovery of the TP biology, and the roles and functions of the receptor. abm has available the A160T mutant of the TP receptor available (Cat. No. T3165).
Procedure Overview
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Over Expression	Thromboxane A2 receptor (TP) via transfection of FLAG-TP-1D4 plasmid
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10% and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C. For membrane protein induction: Grow to 70-80% confluence and treat with tetracycline (1 μg/ml) and sodium butyrate (7.5 mM) to induce the expression of the proteins. For optimum protein production concentration of both tetracycline and sodium butyrate need to be optimized.
Pictures
Pictures
Quality Control	Immunodetection was used to detect TP genetic variant A160T
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.