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KLF4 Lysate

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KLF4 Lysate

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	Print Version
Organism	Human
Accession #	O43474
Alias	EZF, GKLF
Protein Type	Wild Type
Protein Sequence	HHHHHHRQPPGESDMAVSDALLPSFSTFASGPAGREKTLRQAGAPNNRWREELSHMKRLPPVLPGRPYDLAAATVATDLESGGAGAACGGSNLAPLPRRETEEFNDLLDLDFILSNSLTHPPESVAATVSSSASASSSSSPSSSGPASAPSTCSFTYPIRAGNDPGVAPGGTGGGLLYGRESAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVLKASLSAPGSEYGSPSVISVSKGSPDGSHPVVVAPYNGGPPRTCPKIKQEAVSSCTHLGAGPPLSNGHRPAAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYPSFLPDQMQPQVPPLHYQELMPPGSCMPEEPKPKRGRRSWPRKRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRAFSRSDHLALHMKRHFESGGGGSPGRRRRRRRRR
Protein Family	Belongs to the krueppel C2H2-type zinc-finger protein family.
Functions	? Transcription factor which acts as both an activator and repressor. KLF4 binds the CACCC core sequence. It also binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. KLF4 is required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. ? KLF4 is one of the four factors in the Yamanaka protocol for iPSC reprogramming. The purified recombinant protein has a 6X His tag at N-terminus for purification and 9X poly-arginine at C-terminus for cell membrane penetration.
Cell Types	Stem cells
Protein Location	Nucleus
Tag	6X His tag at N-terminus and 9X Poly-Arginine at C-terminus
Form	Liquid
Expression System	E.coli
Type	Lysate
Purification	None
Quality Control	By Western Blot Analysis
Concentration	1mg/ml
Storage	DMEM with 2.5% glycerol
Shipping	On dry ice.
Notes	For Research Use Only, not for therapeutic or diagnostic purposes.
References	Induction of pluripotent stem cells from *** human fibroblasts by defined factors. Takahashi et al. Cell. 2007 Nov 30;131(5):861-72. Induction of pluripotent stem cells from mouse embryonic and *** fibroblast cultures by defined factors. Takahaski and Yamanaka. Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10.