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Immortalized-Mouse-Hepatocyte-SIMH-(CLOCK)-Cells

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Immortalized-Mouse-Hepatocyte-SIMH-(CLOCK)-Cells

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Immortalized-Mouse-Hepatocyte-SIMH-(CLOCK)-Cells

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	Print Version
BioSafety Level	II
Organism	Mouse
Source Organ	Liver
Donor Gender	Male
Growth Properties	Adherent
Morphology	Polygonal
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Immortalization Method	Transfection with mammalian expression vector carrying SV40 gene
Description	The Immortalized Mouse Hepatocyte SIMH^CLOCK Cells contain a bioluminescent marker gene that reports on the circadian phase of the cells. The presence of the Per2:Luc reporter gene allows real time monitoring of clock phase. The major characteristics of this cell line include its high metabolic capacity, liver-like properties, robust synchronizable circadian clock, and capacity to stably express additional marker. When compared with other established cell lines (i.e. HepG2), the SIMH^CLOCK cells show a significantly better metabolic and xenobiotic capacity. This ability of the Immortalized Mouse Hepatocyte SIMH^CLOCK Cells is useful in studying treatment of liver cells with various physical or chemical compounds at defined stages of the circadian cycle and analyze the time-of-day exposure effects.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is PriGrow IX medium available from abm (TM019). To make the completed growth medium, add the following components to the base medium: Fetal Bovine Serum (TM999) to final concentration of 10% and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	1) Bioluminescence recording of luciferase activity; 2) Apoptosis assay using exposure to B[a]P; 3) qPCR; 4) RNA Seq analysis
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.