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$Immortalized-MS<sup>-\/-<\/sup>-Mouse-Alveolar-Macrophage-Cells-(ZK1)$

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Immortalized-MS^{-\/-<\/sup>-Mouse-Alveolar-Macrophage-Cells-(ZK1)}

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Immortalized-MS^{-\/-<\/sup>-Mouse-Alveolar-Macrophage-Cells-(ZK1)}

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	-/--Mouse-Alveolar-Macrophage-Cells-(ZK1)-T0676-Data-Sheet.html" target="_blank" rel="nofollow">Print Version
BioSafety Level	II
Organism	MS^-/- C57BL/6 mice
Source Organ	Brochoalveolar
Growth Properties	Adherent
Morphology	Polygonal
Population Doubling	Doubling time of 14 hours
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	F4/80 and CD11b
Applications	For Research Use Only
Immortalization Method	Infected with J2 retrovirus supernatants collected from the AMJ2-C11 cell line which carries the v-raf and v-myc oncogenes
Description	Alveolar macrophages were isolated from the brochoalveolar lavage fluid of MARCO^-/- SR-AI/II^-/- (MS^-/- ) mice and were immortalized with the J2 retrovirus to establish the Immortalized MS^-/- Mouse Alveolar Macrophage Cells (ZK1). ZK1 cells exhibit the macrophage morphology, functional activity, and the ability to bind to and ingest particles are reduced due to the deficiency in MARCO and SR-AI/II scavenger receptors. It is a valuable tool to study the functions of MARCO and SR-AI/II scavenger receptors and particle toxicity. abm also have available the ZK2 (T0677) and ZK6 (T0678)clones established from limiting dilution.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow II medium available from ABM (TM002). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10% and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	1) PCR genotyping used to determine the deficiency of MARCO and SR-AI/II; 2) Modified Wright staining used to identify macrophage morphology; 3) Macrophage-associated cell surface markers F4/80 and CD11b were determined by flow-cytometry.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.