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$Immortalized-Mouse-CD4<sup>+<\/sup>-CD8<sup>+<\/sup>--T-Cells-(MOHITO)$

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Immortalized-Mouse-CD4^{+<\/sup>-CD8^{+<\/sup>--T-Cells-(MOHITO)}}

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Immortalized-Mouse-CD4^{+<\/sup>-CD8^{+<\/sup>--T-Cells-(MOHITO)}}

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	+-CD8⁺--T-Cells-(MOHITO)-T0131-Data-Sheet.html" target="_blank" rel="nofollow">Print Version
BioSafety Level	II
Organism	Balb/c Mouse
Source Organ	Spleen
Growth Properties	Suspension
Morphology	Round
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Immortalization Method	Spontaneous immortalization
Description	The Immortalized Mouse CD4⁺ CD8⁺ T Cells (MOHITO) has characteristics of an immortalized cell line, but are not immortalized by any immortalization reagents. It expresses the Notch1 and Jak1 mutations, as well as TCR rearrangement, both of which are characteristics to human T-cell acute lymphoblastic leukemia (T-ALL). The cells are derived from CD4⁺ CD8⁺ T cells and are interleukin-7 (IL-7) dependent. The presence of IL-7 and IL-2 are required to activate the JAK/STAT signaling pathway. Transfection with BCR-ABL1 or mutant JAK1 transforms the cells to become IL-7 independent for proliferation. These cells are able to induce T-ALL-like disease when injected into healthy Balb/c mice. This cell line represents a novel model system to study T cell-specific protein signaling and inhibition mechanisms and oncogenic mutations, moreover they can be utilized in in vitro and in vivo pharmaceutical studies.
Procedure Overview
Image
Propagation	Grow cells in 6-well plate with the following conditions. The base medium for this cell line is Prigrow II medium available from ABM (TM002). To make the completed growth medium, add the following components to the base medium: 20% fetal bovine serum (TM999), 10 ng/mL IL-7 (Z200645), 5 ng/mL IL-2 ( Z200137), and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Preservation	Freeze in Prigrow II with 20% fetal bovine serum (TM999) and 5% DMSO
Quality Control	1) Flow cytometry to analyze CD4+ CD8+ phenotype; 2) PCR assay for T cell receptor expression; 3) Sequence analysis to identify point mutations in Notch1 and Jak1
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.