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Immortalized-Baby-Mouse-Kidney-Epithelial-Cells-(iBMK)

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Immortalized-Baby-Mouse-Kidney-Epithelial-Cells-(iBMK)

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Immortalized-Baby-Mouse-Kidney-Epithelial-Cells-(iBMK)

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	Print Version
BioSafety Level	II
Organism	5-day-old wild-type baby C57B/6 mouse
Source Organ	Kidney
Growth Properties	Adherent
Morphology	Cuboidal
Passage Number	5
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers	E-cadherin, CK8 and CK18
Applications	For Research Use Only
Immortalization Method	Serial passaging and electroporation transfection with linearized DNA of E1A and p53DD
Description	The Immortalized Baby Mouse Kidney Epithelial Cells (iBMK, Clone W2) is derived by co-expression of adenovirus E1A and dominant-negative p53 (p53DD). In addition to its non-tumorigenicity, this cell line maintains its primary phenotype and expresses epithelial-specific markers such as E-cadherin, cytokeratin 8 and 18. Together with Bax Knockdown iBMK (T3024), Bak Knockdown iBMK (T3025) and Bax/Bak Double Knockdown iBMK (T3026), these cell lines form the ideal system for studying mechanisms regulating apoptosis. In the same way, when paired with Atg5 Knockdown iBMK (T3027) and Atg7 Knockdown iBMK (T3028), they present an attractive model for investigating the relationship between autophagy and metabolism homeostasis.
Procedure Overview
Image
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from abm (TM003). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10% and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	1. Western blot was used to confirm the presence of E1A and p53DD gene in the immortalized cell line. 2. Western blot was used to check epithelial markers E-cadherin and cytokeratin expression.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.