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Immortalized-Human-Fetal-Osteoblastic-estrogen-receptor-stable-Cell-Line---SV40

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Immortalized-Human-Fetal-Osteoblastic-estrogen-receptor-stable-Cell-Line---SV40

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Immortalized-Human-Fetal-Osteoblastic-estrogen-receptor-stable-Cell-Line---SV40

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	Print Version
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Bone
Growth Properties	adherent
Morphology	polygonal
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Immortalization Method	Conditionally Immortalized with A Temperature-sensitive(ts) mutant, tsA58, of SV40 Large T antigen, stable expressing estrogen receptor
Procedure Overview
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow IV medium available in ABM, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO₂), 5%. Keep cells in G418 selection at 200ug/ml. Temperature: hFOBER cells cultured at the permissive temperature (33.5°C) proliferate rapidly with a doubling time of ~36 hours. hFOB cells cultured at the restrictive temperature (39.5C) do not proliferate, whereas cells cultured at 38.0°C proliferate very slowly, with a doubling time of >96 hours. When the cells are switched back to 33.5°C, proliferation resumes and attains a rate similar to the those grown continuously at 33.5°C.
Quality Control	Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
Reference	Harris SA, Tau KR, Enger RJ, Toft DO, Riggs BL, Spelsberg TC. Estrogen response in the hFOB 1.19 human fetal osteoblastic cell line stably transfected with the human estrogen receptor gene. J Cell Biochem. 1995 Oct;59(2):193-201.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.