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Mouse-Primary-Nephron-Progenitor-Cells

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Mouse-Primary-Nephron-Progenitor-Cells

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Description	The Mouse Primary Nephron Progenitor Cells are isolated from embryonic mouse kidneys. These cells are viable for studies that involve the signaling pathways involved in nephrogenesis and stem/progenitor cell niche within the kidney. They are also applicable in studies of potential stem/progenitor cell regulators as they can easily receive small molecule, recombinant proteins, and viruses.
Procedure Overview
BioSafety Level	II
Organism	E17.5 mouse embryo
Source Organ	Fetal Kidney
Isolation	Limited enzymatic digestion of kidney tissue
Growth Properties	Adherent
Morphology	Cobblestone
Markers	PAX-2
Applications	For Research Use Only
Disease	Normal
Propagation	Use of PriCoat? T25 Flasks (G299) or . Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow X.1 medium available in ABM, Cat. No. TM010. Atmosphere: air, 95%; Carbon dioxide (CO₂), 5%. The cells should be plated on fibronectin coated culture ware to facilitate attachment. The recommended seeding density is 100,000 – 200,000 cells/cm₂.
Preservation	1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control	Immunofluorescence of PAX-2 marker.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.