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HEK293S-TetR GnTI- Stably Expressing A160T Cell Line

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HEK293S-TetR GnTI- Stably Expressing A160T Cell Line

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HEK293S-TetR GnTI- Stably Expressing A160T Cell Line

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	Print Version
BioSafety Level	II
Organism	Homo sapiens
SourceOrgan	Kidney
Growth Properties	Adherent
Morphology	Polygonal
Description	HEK293S-TetR GnTI Stably Expressing A160T Cell line was established to have a tetracycline-inducible HEK293S stable cell line which expresses the A160T mutant of the thromboxane A2 receptor (TP). In which the genetic variant exhibited loss of thermal stability when compared to the wild type, hence some loss of activity or thermal sensitivity.. Additionally, the HEK293S-TetR GnTI- cells do not express the N-acetylglucosaminyltransferase I (GnTI).TP is a G-protein coupled receptor (GPCR) involved in vasoconstriction and thrombosis. It is valuable tool for discovery of the TP biology, and the roles and functions of the receptor. abm has available the wild type of the TP receptor available (Cat. No. T3163).
Procedure Overview
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Over Expression	Thromboxane A2 receptor (TP) via transfection of FLAG-TP-A160T-1D4 plasmid
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10% and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C. For membrane protein induction: Grow to 70-80% confluence and treat with tetracycline (1 μg/ml) and sodium butyrate (7.5 mM) to induce the expression of the proteins. For optimum protein production concentration of both tetracycline and sodium butyrate need to be optimized.
Quality Control	Immunodetection was used to detect TP genetic variant A160T
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.