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Immortalized-Mouse-Preadipocyte-Cells-(ScAP-23)

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Immortalized-Mouse-Preadipocyte-Cells-(ScAP-23)

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Immortalized-Mouse-Preadipocyte-Cells-(ScAP-23)

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	Print Version
BioSafety Level	II
Organism	Mouse
Source Organ	Adipose tissue
Growth Properties	Adherent
Morphology	Fibroblast-like
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Immortalization Method	Serial passaging and infection with retroviruses carrying hTERT gene
Description	The Immortalized Mouse Preadipocyte Cells (ScAP-23) was isolated from subcutaneous adipose tissue from C57BL/6 mice and immortalized by infection with retroviruses carrying hTERT gene. The ScAP-23 preadipocytes convert to adipocytes when treated with dexamethasone, 3-methylisobutylxanthine, insulin, and indomethacin. Expression of key adipogenic transcription factors such as PPAR?, C/EBP family, SREBP-1c transcription factors and Krox20/Egr2 are found upregulated during ScAP-23 adipogenesis. The ScAP-23 adipocytes contain abundant lipid droplets and express transcripts for mature adipocytes markers such as SCD1, aFABP, ATGL, FAS, LDL, and GPDH. Furthermore, ScAP-23 adipocytes demonstrate insulin responsiveness and express insulin receptor-β and major insulin-responsive glucose transporter GLUT4. Thus, the cell line is recommended as a tool for adipocytes biology and adipogenesis research.
Procedure Overview
Image
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. Grow cells in T25 ECM-coated flasks (G299) with the following conditions. The base medium for this cell line is PriGrow III medium (TM003). To make the completed growth medium, add the following components to the base medium at a final concentration of 10% fetal bovine serum (TM999), 2mM L-glutamine (G275), and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	1) Oil Red O staining at day 7 after inducing adipocyte differentiation to visualize lipid droplets; 2) Harvest RNA after induction of adipogenesis and perform RT-PCR and Northern Blot to detect transcript level of adipocyte markers SCD1, aFABP, ATGL, FAS, LDL, and GPDH B.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.