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Immortalized-Mouse-Distal-Tubule-KPT2-Cells

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Immortalized-Mouse-Distal-Tubule-KPT2-Cells

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Immortalized-Mouse-Distal-Tubule-KPT2-Cells

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	Print Version
BioSafety Level	II
Organism	Mouse
Source Organ	Kidney
Growth Properties	Adherent
Morphology	Fibroblast-like
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Immortalization Method	Spontaneously immortalized in culture
Description	Immortalized Mouse Proximal Tubule KPT2 Cells are spontaneously immortal derived cells from murine kidney. It does not express high levels of the Ca²⁺-permeable non-selective cation channel, TRPV4, unlike the Immortalized Mouse Distal Tubule KDT3 Cells (abm, T0461). However, expresses SGLT2, an electrogenic sodiumglucose transporter-2, which may have a role in gentamicin-induced toxicity. KPT2 cells may be used to assess the effects of the presence of SGLT2 and the absence of TRPV4 has on aminoglycosides uptake in vitro. Additionally, these cells are the wild type to the Stable TRPV4 Expressing KPT2 Cell Line (abm, T6009).
Procedure Overview
Image
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from ABM (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10% and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	1) RT-PCR was used to detect the presence or absence of TRPV4 transcript, 2) Immunofluorescence was utilized to assess the presence of SGLT2.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.