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Bovine-Primary-Lymphocytes

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Bovine-Primary-Lymphocytes

商品詳情參考文獻(xiàn) 相關(guān)資料

	Print Version
Procedure Overview
BioSafety Level	II
Organism	Bovine
Source Organ	Retropharyngeal lateral lymph node
Growth Properties	Suspension
Morphology	Spheric
Recommended Seeding Density	1-2 x 10⁶ cells/ml
Applications	For Research Use Only
Disease	Normal
Propagation	The base medium for this cell line is Prigrow II medium available in abm, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: 10% heat-inactivated fetal bovine serum, 1mM MEM sodium pyruvate, 50uM 2-mercaptoethanol, 1X Hybridoma Fusion and Cloning Supplement (HFCS, Roche 11363735001) and Penicillin/Streptomycin (G255). Add HFCS fresh. Atmosphere: air, 95%; Carbon dioxide (CO₂), 5%. The Bovine Primary Lymphocytes do not proliferate in vitro , but can survive in culture for a few days.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.