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Human Primary Retinal Pigment Epithelial Cells

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Human Primary Retinal Pigment Epithelial Cells

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Human Primary Retinal Pigment Epithelial Cells

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Description	Human Retinal Pigment Epithelial Cells are major components of the human retina, a multi-layered lining of the rear surface of the eye. They are different from many other types of epithelia in the polarity of a number of proteins, and their apical plasma membrane is in contact with the extracellular matrix rather than devoid of matrix like other epithelial cell types. They are critical in the development and maintenance of adjacent photoreceptors in retina, playing a key role in maintaining normal vision and supports photo-transduction due to its anatomic location between the photoreceptors and the choriocapillaries. These cells are ideal models for research on ocular diseases and study of cell-matrix interactions, gene regulation and tissue development.
Procedure Overview
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Retina
Growth Properties	Adherent
Morphology	Polygonal
Image
Passage Number	2
Population Doubling	10 PD. Doubling time: 27.85 hours
Markers	CK-18, CK-19, fibronectin
Applications	For Research Use Only
Disease	Normal
Propagation	Use of PriCoat? T25 Flasks (G299) or . Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow I medium available in ABM, Cat. No. TM001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO₂), 5%.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.