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$Immortalized-Human-Foreskin-Keratinocytes-(16E6\/E7-HFK)$

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Immortalized-Human-Foreskin-Keratinocytes-(16E6\/E7-HFK)

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Immortalized-Human-Foreskin-Keratinocytes-(16E6\/E7-HFK)

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	Print Version
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Foreskin tissue
Donor Age	Neonatal
Growth Properties	Adherent
Morphology	Polygonal
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Immortalization Method	Transfection with retrovirus expressing HPV-16 E7 and HPV-16 E6
Description	The Immortalized Human Foreskin Keratinocytes (16E6/E7 HFK) were derived from foreskin tissue of newborns and were transfected with a retrovirus expressing HPV-16 E7 and HPV-16 E6. The HPV-16 E6 and E7 delay cellular differentiation and keep the immortalized cells in a state of DNA synthesis even after they have entered the suprabasal layer of the epidermis. Serum-induced and calcium-induced differentiation of the Immortalized Human Foreskin Keratinocytes is also inhibited by HPV-16 E6. In these immortalized cells, the regulated genes by the HPV-16 E6 and E7 may play a role in the HPV life cycle and in HPV-mediated carcinogenesis which make the Immortalized Human Foreskin Keratinocytes (16E6/E7 HFK) valuable not only as a model in immunology but also in gene regulation studies.
Procedure Overview
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Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The growth medium for this cell line is Keratinocyte-SFM (Gibco-BRL, Rockville, MD) with 1% Penicillin/Streptomycin Solution available from abm (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	1) ELISA to measure telomerase activity; 2) Microarray analysis of gene regulation
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.