欧美成人精品三区综合A片,国产九九av

您好，歡迎來到拜力生物網(wǎng)站！請登錄注冊

我的訂單 | 聯(lián)系我們 | 在線客服QQ：3012681483 | 客服電話：400-968-7988 | 工作時間：08:30-17:30

主營產(chǎn)品： ELISA試劑盒、抗體、補體血清、兔補體、干擾素、小鼠測溫儀、進口試劑、Quidel代理、Swant代理、PBL Assay Science代理

網(wǎng)站首頁產(chǎn)品中心代理品牌新聞資訊資源中心關于我們

所在位置：首頁 > 產(chǎn)品中心 > abmgood > abmgood產(chǎn)品

Immortalized-Human-Bronchial-Epithelial-Cells-(HBE1)-

訂購信息

上海拜力生物科技公司

Tel:400-968-7988 021-33779008

Immortalized-Human-Bronchial-Epithelial-Cells-(HBE1)-

品牌：Abmgood

貨號：

規(guī)格：

貨期：

應用：

咨詢客服

立即購買加入購物車

Immortalized-Human-Bronchial-Epithelial-Cells-(HBE1)-

商品詳情參考文獻相關資料

	Print Version
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Bronchi
Donor Age	60 y/o
Donor Gender	Female
Growth Properties	Adherent
Morphology	Epithelial-like
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Immortalization Method	Transfection with the pUC19 plasmid encoding intact HPV-18 E6 and E7 gene
Description	Immortalized Human Bronchial Epithelial Cells (HBE1) is derived from a 60 year-old female donor suffering from idiopathic pulmonary fibrosis and was immortalized with HPV-18 E6/E7. The cells retain ion transport properties and express the cystic fibrosis transmembrane conductance regulator (CFTR) protein. It is a valuable tool for studies relating to cystic fibrosis alongside with the Immortalized Human Cystic Fibrosis Tracheobronchial Epithelial Cells (CFT1) (available at abm,T0681). It can also be used for research in human lung biology, and mechanisms involved.
Procedure Overview
Image
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow IV medium available from abm (TM004). To make the completed growth medium, add the following components to the base medium in the specified final concentrations: 10 μg/ml insulin (), 10^-6M hydrocortisone, 3.75 μg/ml endothelial cell growth supplement (Sigma; E2759), 25 ng/ml EGF (), 3x10^-8M T3 (triiodothyronine) (Sigma; T06397), 5 μg/ml Transferrin (Sigma; T0665), 10 ng/mlCholera toxin (Sigma; C-3012), and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	Immunoblots were utilized to confirm the presence of CFTR
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.